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- Title
Integration of Retrotransposons-Based Markers in a Linkage Map of Barley.
- Authors
Rodriguez, Monica; O'Sullivan, Donal; Donini, Paolo; Papa, Roberto; Chiapparino, Elena; Leigh, Fiona; Attene, Giovanna
- Abstract
A deeper understanding of random markers is important if they are to be employed for a range of objectives. The sequence specific amplified polymorphism (S-SAP) technique is a powerful genetic analysis tool which exploits the high copy number of retrotransposon long terminal repeats (LTRs) in the plant genome. The distribution and inheritance of S-SAP bands in the barley genome was studied using the Steptoe × Morex (S × M) double haploid (DH) population. Six S-SAP primer combinations generated 98 polymorphic bands, and map positions were assigned to all but one band. Eight putative co-dominant loci were detected, representing 16 of the mapped markers. Thus at least 81 of the mapped S-SAP loci were dominant. The markers were distributed along all of the seven chromosomes and a tendency to cluster was observed. The distribution of S-SAP markers over the barley genome concurred with the knowledge of the high copy number of retrotransposons in plants. This experiment has demonstrated the potential for the S-SAP technique to be applied in a range of analyses such as genetic fingerprinting, marker assisted breeding, biodiversity assessment and phylogenetic analyses.
- Subjects
TRANSPOSONS; BARLEY; GENETIC polymorphisms; MOBILE genetic elements; PLANT genetics
- Publication
Molecular Breeding, 2006, Vol 17, Issue 2, p173
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-005-4885-4