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- Title
Surface- engineered Saccharomyces cerevisiae displaying α-acetolactate decarboxylase from Acetobacter aceti ssp xylinum.
- Authors
Cejnar, Rudolf; Dostálek, Pavel; Hložková, Kateřina; Kotrba, Pavel
- Abstract
Objectives: To convert α-acetolactate into acetoin by an α-acetolactate decarboxylase (ALDC) to prevent its conversion into diacetyl that gives beer an unfavourable buttery flavour. Results: We constructed a whole Saccharomyces cerevisiae cell catalyst with a truncated active ALDC from Acetobacter aceti ssp xylinum attached to the cell wall using the C-terminal anchoring domain of α-agglutinin. ALDC variants in which 43 and 69 N-terminal residues were absent performed equally well and had significantly decreased amounts of diacetyl during fermentation. With these cells, the highest concentrations of diacetyl observed during fermentation were 30 % less than those in wort fermented with control yeasts displaying only the anchoring domain and, unlike the control, virtually no diacetyl was present in wort after 7 days of fermentation. Conclusions: Since modification of yeasts with ALDC variants did not affect their fermentation performance, the display of α-acetolactate decarboxylase activity is an effective approach to decrease the formation of diacetyl during beer fermentation.
- Subjects
ACETOLACTATE synthase; DECARBOXYLASES; ACETOBACTER aceti; ACETOIN; SACCHAROMYCES cerevisiae; DIACETYL; YEAST fungi biotechnology; BEER flavor &; odor
- Publication
Biotechnology Letters, 2016, Vol 38, Issue 12, p2145
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-016-2205-1