We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Inhibition of autophagy by autophagic inhibitors enhances apoptosis induced by bortezomib in non-small cell lung cancer cells.
- Authors
Wu, Guodong; Li, Haifeng; Ji, Zhiyong; Jiang, Xiaoming; Lei, Yu; Sun, Mingli
- Abstract
Bortezomib is a novel proteasome inhibitor that has promising antitumor activity against various cancer cells. We have assessed its antitumor activity in non-small cell lung cancer (NSCLC) A549 and H157 cells in vitro where it inhibited cell growth and induced apoptosis, which was associated with cytochrome c release and caspase-3 activation. Bortezomib upregulated autophagic-related proteins, the Atg12-Atg5 complex and LC3-II, which indicated autophagy had occurred. The combination of bortezomib with autophagic inhibitor 3-methyladenine or chloroquine significantly enhanced suppression of cell growth and apoptosis induced by bortezomib in A549 and H157 cells. Our study indicated that inhibition of both proteasome and autophagy has great potential for NSCLC treatment.
- Subjects
LUNG cancer treatment; BORTEZOMIB; CHLOROQUINE; APOPTOSIS; AUTOPHAGY; PROTEASOME inhibitors; ANTINEOPLASTIC agents; THERAPEUTICS
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 6, p1171
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1470-0