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- Title
Detection of drug transporter expression using a 25-multiplex RT-PCR assay.
- Authors
Lee, Tae-Bum; Choi, Cheol-Hee
- Abstract
Membrane transporters play important roles in mediating chemoresistance and chemosensitivity of tumor cells. Five sets of 5 genes were designed to simultaneously detect drug transporter expression in a single reaction tube using multiplex RT-PCR for 25 genes, including 17 ABC transporters, one non-ABC transporter, 4 SLC transporters, 2 copper transporters and one housekeeping gene. We optimized the multiplex RT-PCR conditions using chemosensitive cancer cells and then validated the system using chemoresistant cancer cells. This reliable multiplex RT-PCR assay can be utilized not only to investigate anticancer drug-resistance mechanisms but also to estimate the efficacy of anticancer chemotherapy in the clinic.
- Subjects
BIOLOGICAL transport; GENES; CANCER cells; DRUG resistance; MULTIPLEXING
- Publication
Biotechnology Letters, 2009, Vol 31, Issue 10, p1485
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-009-0039-9