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- Title
Isolation and characterization of a metallothionein-1 protein in Chloris virgata Swartz that enhances stress tolerances to oxidative, salinity and carbonate stress in Saccharomyces cerevisiae.
- Authors
Nishiuchi, Shunsaku; Shenkui Liu; Takano, Tetsuo
- Abstract
Chloris virgata Swartz ( C. virgata) is a gramineous wild plant that is found in alkaline soil areas in northeast China and is highly tolerant to carbonate stress. We constructed a cDNA library from C. virgata seedlings treated with NaHCO3, and isolated a type1 metallothionein (MT1) gene ( ChlMT1: AB294238) from the library. The amino acid sequence of ChlMT1 contained 12 cysteine residues that constituted the Cys-X-Cys (X = amino acid except Cys) motifs in the N- and C-terminal regions. Northern hybridization showed that expression of ChlMT1 was induced by several abiotic stresses, from salts (NaCl and NaHCO3), a ROS inducer (paraquat), and metals (CuSO4, ZnSO4, and CoCl2). ChlMT1 expression in leaf was induced by 200 mM NaCl and 100 mM NaHCO3. About 5 μM Paraquat, 500 μM Zn2+, and 500 μM Co2+ also induced expression of ChlMT1 in leaf after 6 h, and 100 μM Cu2+ induced it after 24 h. Saccharomyces cerevisiae when transformed with the ChlMT1 gene had dramatically increased tolerances to salts (NaCl and NaHCO3) and ROS.
- Subjects
METALLOTHIONEIN; EDIBLE wild plants; CYSTEINE proteinases; AMINO acid sequence; NUCLEIC acid hybridization
- Publication
Biotechnology Letters, 2007, Vol 29, Issue 8, p1301
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-007-9396-4