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- Title
Phenotypic Effects and Inhibition of Botrydial Biosynthesis Induced by Different Plant-Based Elicitors in <italic>Botrytis cinerea</italic>.
- Authors
Liñeiro, Eva; Macias-Sánchez, Antonio J.; Espinazo, Marisa; Cantoral, Jesús M.; Moraga, Javier; Collado, Isidro G.; Fernández-Acero, Francisco J.
- Abstract
<italic>Botrytis cinerea</italic> is considered a model organism for the study of plant-pathogen interaction showing great genetic diversity and a high degree of morphological variability depending on environmental conditions. The use of new compounds and plant-based elicitors may trigger the expression of different <italic>B. cinerea</italic> genes, providing new sources of virulence factors. This work is focused on elucidating the phenotypic effect in <italic>B. cinerea</italic> of different carbon sources such as glucose, cellulose and tomato cell walls (TCW). Production of botrydial and dihydrobotrydial toxins was evaluated using thin-layer chromatography (TLC), proton nuclear magnetic resonance spectroscopy (1H-NMR) and mass spectrometry (UPLC-HRESIMS). Expression of the toxin biosynthesis gene <italic>BcBOT2</italic> was followed using RT-qPCR. Results show an inhibition of the toxin biosynthesis pathway when TCW are present as a sole carbon source, suggesting that the toxin is only produced when rich molecules, like glucose, are available for fungal metabolism. That suggests a connection between gene expression of virulence factors and environmental conditions, where the silent genes can be induced by different culture conditions.
- Subjects
BOTRYTIS cinerea; ELICITORS (Botany); PLANT-pathogen relationships; PROTON magnetic resonance spectroscopy; PHYTOTOXINS
- Publication
Current Microbiology, 2018, Vol 75, Issue 4, p431
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-017-1399-3