We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A gene associated with filamentous growth in Ophiostoma novo-ulmi has RNA-binding motifs and is similar to a yeast gene involved in mRNA splicing.
- Authors
Pereira, Veronica; Royer, John C.; Hintz, William E.; Field, Deborah; Bowden, Christine; Kokurewicz, Karl; Hubbes, Martin; Horgen, Paul A.
- Abstract
The COL1 gene was isolated from Ophiostoma novo-ulmi using the techniques of insertional mutagenesis and plasmid rescue. Sequence analyses suggest that the COL1 gene encodes a unique protein of 826 amino acids with consensus-type RNA-binding domains, most similar to a putative protein from Schizosaccharomyces pombe which resembles the C-terminus of the Saccharomyces cerevisiae U4/U6 splicing factor PRP24. Disruption of the COL1 gene produced the yeast-like col1 mutant. The inability of the mutant to synthesize the COL1 gene product was confirmed by transcript analysis. Transformation of the col1 mutant with the COL1 gene restored the wild phenotype and production of the 4.0-kb mRNA. The results from this study demonstrate that the COL1 RNA-binding protein is associated with filamentous growth in O. novo-ulmi.
- Subjects
GENES; NUCLEIC acids; RNA; OPHIOSTOMA; MESSENGER RNA; GENETIC mutation; AMINO acids
- Publication
Current Genetics, 2000, Vol 37, Issue 2, p94
- ISSN
0172-8083
- Publication type
Article
- DOI
10.1007/s002940050015