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- Title
Investigation of the Effectiveness of Chromogenic Media in the Isolation of Pasteurella multocida and Mannheimia haemolytica from Calf Nasal Swab Samples.
- Authors
TEL, Osman Yaşar; ÖTKÜN, Songül; YÜCETEPE, Ayfer Güllü; KESKİN, Oktay
- Abstract
Accurate and rapid diagnosis of disease agents is the most important step in terms of control practices. Chromogenic media are culture media that allow the formation of colonies in colors specific to target microorganisms. Because they are target-specific, they do not require validation of results but provide ease of use and time savings. In this study, it was aimed to evaluate the effectiveness of a chromogenic medium (Pasteurella BDR kit) in the detection of Pasteurella multocida and Mannheimia haemolytica agents in the Pasteurelleceae family, which cause respiratory disease in cattle. In this study, nasal swab samples taken from calves showing symptoms of respiratory disease were cultured in chromogenic and standard media. Suspicious growing colonies were confirmed by polymerase chain reaction for P. multocida and M. haemolytica. While 31 (36.9%) samples formed colonies with the chromogenic medium in the color specific to the target bacteria, 28 (33.3%) samples were positively determined for the target bacteria using the standard cultural method. The results of 26 samples were positive by both cultural diagnosis methods. When the results were compared with the traditional cultural diagnosis, agreement was found to be 92.86%. All colored colonies grown on the chromogenic medium were also tested by polymerase chain reaction (PCR). It was determined that the chromogenic medium detected P. multocida at a rate of 92.86% (n = 26) and M. haemolytica at a rate of 100% (n = 2) by forming colonies with a family-specific color. As a result, it was concluded that the use of chromogenic media is beneficial in the practical, rapid, and high-accuracy diagnosis of target agents.
- Subjects
MANNHEIMIA haemolytica; PASTEURELLA multocida; POLYMERASE chain reaction; CALVES; SYMPTOMS; CATTLE diseases; RESPIRATORY diseases
- Publication
Veterinary Sciences & Practices, 2022, Vol 17, Issue 3, p81
- ISSN
2822-3608
- Publication type
Article
- DOI
10.5152/VetSciPract.2022.221830