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- Title
Otimização das condições de ensaio in vivo da enzima redutase do nitrato em Cucurbitáceas.
- Authors
RODRIGUES, Biank Amorim; MESQUITA, Alessandro Carlos
- Abstract
Cucurbits include species of importance to the Northeast of Brazil, among them melon, watermelon and cucumber are those that have the most expressive cultivation. The production system of these vegetables requires the presence of nitrogen, which, whereas the reduction of nitrate through the activity of nitrate reductase in the cell's cytosol is an important part of the process of incorporating N into amino acids. However, the methodologies used are diverse and applied in different cultures in general, with the need for greater specificity. The present study aimed to standardize the most appropriate conditions for determining the activity of RN, in vivo, in the leaf tissue of watermelon, melon and cucumber. The experiment was conducted in a completely randomized design in a 3x3 factorial, comprising three in vivo methodologies and three proposals for infiltration of plant material (water bath, greenhouse and vacuum), with four replications. The plants were conducted in a greenhouse and the fully expanded leaf tissues were collected, after 20 days of germination, to conduct the enzymatic methods and evaluate the activity of the nitrate reductase (NR) enzyme. The results obtained demonstrated that the greatest activity of NR in the watermelon and melon leaf tissue was obtained using Methodology 1 submitted to vacuum, while for cucumber the best test was Methodology 2, when also submitted to vacuum.
- Publication
Nativa, 2021, Vol 9, Issue 1, p9
- ISSN
2318-7670
- Publication type
Article
- DOI
10.31413/nativa.v9i1.10546