We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Concomitant activation of the PI3K/Akt and ERK1/2 signalling is involved in cyclic compressive force-induced IL-6 secretion in MLO-Y4 cells.
- Authors
Yin, Jian; Hao, Zhichao; Ma, Yuanyuan; Liao, Shuang; Li, Xianxian; Fu, Jing; Wu, Yeke; Shen, Jiefei; Zhang, Ping; Li, Xiaoyu; Wang, Hang
- Abstract
IL-6 has a dual role in bone remodelling. The ERK1/2 pathway partially upregulated IL-6 secretion in osteocyte-like MLO-Y4 cells exposed to CCF. We have now investigated the possible role of phosphatidylinositol 3-kinase (PI3K)/Akt signalling pathway in the CCF-induced IL-6 expression. MLO-Y4 cells were treated with CCF 2,000 µstrain, 2 Hz, or 10, 30 min, 1, 3 and 6 h. IL-6 expression, Akt and ERK1/2 and PI3K/Akt phosphorylation were determined by RT-PCR, ELISA and Western blotting. Inhibition of PI3K/Akt with LY294002 or ERK1/2 with PD98059 significantly attenuated IL-6 upregulation, and IL-6 expression was abolished by inhibiting both pathways. Inhibition of one pathway downregulated the other's phosphorylation level. In conclusion, concomitant activation of PI3K/Akt and ERK1/2 pathways mediated IL-6 expression in MLO-Y4 cells under CCF.
- Subjects
PHOSPHATIDYLINOSITOL 3-kinases; INTERLEUKIN-6; WESTERN immunoblotting; POLYMERASE chain reaction; ENZYME-linked immunosorbent assay
- Publication
Cell Biology International, 2014, Vol 38, Issue 5, p591
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.10235