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- Title
De novo formation of basal bodies in Naegleria gruberi: regulation by phosphorylation.
- Authors
Hong-Kyung Kim; Jeong-Gu Kang; Shigehiko Yumuro; Walsh, Charles J.; Jin Won Cho; JooHun Lee
- Abstract
The de novo formation of basal bodies in Naegleria gruberi was preceded by the transient formation of a microtubule (MT)-nucleating complex containing &#x03B3-tubulin, pericentrin, and myosin II complex (GPM complex). The MT-nucleating activity of GPM complexes was maximal just before the formation of visible basal bodies and then rapidly decreased. The regulation of MT-nucleating activity of GPM complexes was accomplished by a transient phosphorylation of the complex. Inhibition of dephosphorylation after the formation of basal bodies resulted in the formation of multiple flagella. 2D-gel electrophoresis and Western blotting showed a parallel relationship between the MT-nucleating activity of GPM complexes and the presence of hyperphosphorylated &#x03B3-tubulin in the complexes. These data suggest that the nucleation of MTs by GPM complexes precedes the de novo formation of basal bodies and that the regulation of MT-nucleating activity of GPM complexes is essential to the regulation of basal body number.
- Subjects
NAEGLERIA gruberi; NAEGLERIA; PHOSPHORYLATION; MICROTUBULES; MATHEMATICAL complexes; FLAGELLA (Microbiology)
- Publication
Journal of Cell Biology, 2005, Vol 169, Issue 5, p719
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200410052