We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The blood serum metabolome profile after different phases of a 4‐km cycling time trial: Secondary analysis of a randomized controlled trial.
- Authors
Azevedo, Rafael A.; Cruz, Ramon; Silva‐Cavalcante, Marcos D.; Lima‐Silva, Adriano E.; Bertuzzi, Romulo
- Abstract
It has been assumed that exercise intensity variation throughout a cycling time trial (TT) occurs in alignment of various metabolic changes to prevent premature task failure. However, this assumption is based on target metabolite responses, which limits our understanding of the complex interconnection of metabolic responses during exercise. The current study characterized the metabolomic profile, an untargeted metabolic analysis, after specific phases of a cycling 4‐km TT. Eleven male cyclists performed three separated TTs in a crossover counterbalanced design, which were interrupted at the end of the fast‐start (FS, 600 ± 205 m), even‐pace (EP, 3600 ± 190 m), or end‐spurt (ES, 4000 m) phases. Blood samples were taken before any exercise and 5 min after exercise cessation, and the metabolomic profile characterization was performed using Nuclear Magnetic Resonance metabolomics. Power output (PO) was also continually recorded. There were higher PO values during the FS and ES compared to the EP (all p < 0.05), which were accompanied by distinct metabolomic profiles. FS showed high metabolite expression in TCA cycle and its related pathways (e.g., glutamate, citric acid, and valine metabolism); whereas, the EP elicited changes associated with antioxidant effects and oxygen delivery adjustment. Finally, ES was related to pathways involved in NAD turnover and serotonin metabolism. These findings suggest that the specific phases of a cycling TT are accompanied by distinct metabolomic profiles, providing novel insights regarding the relevance of specific metabolic pathways on the process of exercise intensity regulation. Highlights: Untargeted metabolomic profile analysis may reveal unexplored bioenergetic pathways activated throughout a self‐paced cycling time trial.Exercise intensity variations throughout a cycling time trial are accompanied by different blood serum metabolomic profiles.The current new insights may encourage future investigation on the underpinning mechanisms of specific metabolomic profiles according to each time trial phase.
- Subjects
BRAZIL; BLOOD serum analysis; BLOOD testing; VALINE metabolism; GLUTAMIC acid metabolism; EXERCISE physiology; WORK measurement; SECONDARY analysis; RESEARCH funding; NUCLEAR magnetic resonance spectroscopy; EXERCISE intensity; DESCRIPTIVE statistics; CYCLING; CROSSOVER trials; MASS spectrometry; METABOLOMICS; BODY movement; CITRATES; SEROTONIN; EVALUATION
- Publication
European Journal of Sport Science, 2024, Vol 24, Issue 6, p721
- ISSN
1746-1391
- Publication type
Article
- DOI
10.1002/ejsc.12108