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- Title
Effect of water temperature on the development of Portunus trituberculatus larvae in the off-season breeding mode (Decapoda, Portunidae).
- Authors
Wu, Jialin; Wan, Litao; Li, Bin; Zhang, Dongxu; Shi, Huilai; Zhang, Tao; Ping, Hongling; He, Jie
- Abstract
To clarify the influence of different water temperatures on the development of Portunus trituberculatus (swimming crab) larvae during off-season (early autumn) breeding, a gradient of four rearing temperatures of 27, 29, 31 and 33°C was set up, and the effects of water temperature on larval development were systematically compared. The results showed that the moulting cycle (MC) from the zoea 1 (Z1)-zoea 2 (Z2) stage was not significantly affected by water temperature (P > 0.05), remaining at approximately 3 days. However, in the Z2-megalopa (M) stage, the MC in the 31°C group was consistently the shortest. The total developmental duration and total effective cumulative temperature during Z1-M in the 31°C group were the lowest at 9.97 days and 250 days · °C, respectively. According to the equation of water temperature and larval development rate, the development rate was the fastest when the water temperature was 31°C. As the water temperature increased, the survival rate (SR) of larvae at various stages gradually decreased. The SR of the 27°C group was consistently significantly higher than that of the 33°C group (P < 0.05), especially in the M stage, where the SR of the 33°C group was only 2%, which was significantly lower than that of the 27°C and 29°C groups (P < 0.05). Among the antioxidant indexes, both superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) were the lowest in the 29°C group at 315.75 U/mg protein and 0.36 mmol/g protein, respectively, and both were significantly lower than those in the 33°C group (P < 0.05). Catalase (CAT) and glutathione peroxidase (GSH-PX) were both the lowest in the 31°C group, and in this group the CAT was significantly lower than in the 33°C group, and GSH-PX was significantly lower than in the 27°C group (P < 0.05). For malondialdehyde (MDA) content, the four groups were basically maintained at about 2.5 nmol/mg protein, and there was no significant difference (P > 0.05). In terms of digestive enzyme activity, the activity of lipase (LPS) was basically maintained at 1.93 U/g protein. The other four digestive enzyme activity differences were mainly reflected in that the 31 and 33°C groups maintained relatively higher levels than the 27 and 29°C groups, especially the activities of α -amylase (AMS), and cellulase (CL) in the 33°C group, which were significantly higher than those in the 27 and 29°C groups (P < 0.05). Comprehensive indicators indicate that a water temperature between 29 and 31°C is the appropriate temperature for the development of the larvae of off-season breeding swimming crabs.
- Subjects
WATER temperature; PORTUNUS; PORTUNIDAE; GLUTATHIONE peroxidase; DECAPODA; TEMPERATURE effect; DIGESTIVE enzymes
- Publication
Crustaceana, 2024, Vol 97, Issue 3/4, p201
- ISSN
0011-216X
- Publication type
Article
- DOI
10.1163/15685403-bja10350