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- Title
Cytokine mRNA profiles in Epstein–Barr virus-associated post-transplant lymphoproliferative disorders.
- Authors
Nalesnik, Michael A; Zeevi, Adriana; Randhawa, Parmjeet S; Faro, Albert; Spichty, Kathy J; Demetris, Anthony J; Fung, John J; Whiteside, Theresa L; Starzl, Thomas E
- Abstract
Cytokine mRNA patterns were analyzed in 11 post-transplant lymphoproliferative disorder (PTLD) specimens using qualitative reverse-transcriptase polymerase chain reaction (RT-PCR). In each case, a pattern of IL2-, IFNΓ-, IL4+, IL10+ was seen. A similar pattern was observed in a spleen sample from 1 patient with contemporaneous PTLD elsewhere. Semiquantitative RT-PCR for cytokine message was performed using RNA from bronchoalveolar lavage (BAL) specimens obtained from 2 patients with pulmonary PTLD. In both cases, IL4 message predominated. Reduction of message coincided with resolution of the tumors. The pattern differed from that seen in 1 patient with acute pulmonary rejection, in which RT-PCR of BAL cells showed predominance of IL6 and IFNΓ. We conclude that at least some PTLDs exist within a T-helper cell type 2 (Th2)-like cytokine microenvironment. The presence of a similar mRNA pattern in an extratumoral specimen at the time of PTLD suggests that it may reflect a systemic phenomenon. Disappearance of this pattern following PTLD resolution indicates its dynamic nature and is consistent with the hypothesis that specific cytokines contribute to the development of PTLDs.
- Subjects
CYTOKINES; TRANSPLANTATION of organs, tissues, etc.; LYMPHOPROLIFERATIVE disorders
- Publication
Clinical Transplantation, 1999, Vol 13, Issue 1, p39
- ISSN
0902-0063
- Publication type
Article
- DOI
10.1034/j.1399-0012.1999.t01-2-130106.x