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- Title
Whole cell bioconversion of (+)-valencene to (+)-nootkatone by Yarrowia lipolytica using a three phase partitioning bioreactor.
- Authors
Palmerín‐Carreño, Dulce M; Castillo‐Araiza, Carlos O; Rutiaga‐Quiñones, Olga M; Verde Calvo, José R; Trejo‐Aguilar, Gloria M; Dutta, Abhishek; Huerta‐Ochoa, Sergio
- Abstract
BACKGROUND Low permeability of substrates across the cell membrane, cofactor regeneration and product inhibition are some drawbacks of (+)-nootkatone bioconversion. The aim of this work was to evaluate and enhance the bioconversion of (+)-valencene to (+)-nootkatone with Yarrowia lipolytica in a partitioning bioreactor using orange essential oil as the dispersed phase. RESULTS Preliminary experiments in shake flasks allowed enhancing (+)-nootkatone bioconversion to obtain favorable operating conditions (0.2% w/v of CTAB, 2.0 mmol L−1 of niacin and 11.5 g L−1 of biomass) to produce 420.9 mg L−1. Bioreactor experiments in a two-phase system using 0.2% (w/v) of CTAB, 2.0 mmol L−1 of niacin and 22.5 g L−1 of biomass produced a maximum (+)-nootkatone concentration of 619.8 mg L−1 which was around the product inhibition concentration. Nevertheless, the partitioning three-phase system using orange essential oil overcame product inhibition, obtaining concentrations up to 852.3 mg L−1. CONCLUSIONS This is the first report of a wild type Y. lipolytica with the enzymatic machinery to carry out this bioconversion. The multiphase partitioning bioreactor concept seems to have good potential for enhancing the productivity of (+)-nootkatone. The bioconversion approach presents an attractive way to produce and recover (+)-nootkatone in situ using a natural (+)-valencene source. © 2015 Society of Chemical Industry
- Subjects
BIOCONVERSION; NOOTKATONE; PHASE partition; BIOREACTORS; BIOMASS production; FUNGAL membranes
- Publication
Journal of Chemical Technology & Biotechnology, 2016, Vol 91, Issue 4, p1164
- ISSN
0268-2575
- Publication type
Article
- DOI
10.1002/jctb.4702