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- Title
The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel.
- Authors
Müller, Heike M.; Schäfer, Nadine; Bauer, Hubert; Geiger, Dietmar; Lautner, Silke; Fromm, Jörg; Riederer, Markus; Bueno, Amauri; Nussbaumer, Thomas; Mayer, Klaus; Alquraishi, Saleh A.; Alfarhan, Ahmed H.; Neher, Erwin; Al‐Rasheid, Khaled A. S.; Ache, Peter; Hedrich, Rainer
- Abstract
Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss?, Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P. dactylifera., To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid ( ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The Phoenix SLAC1-type anion channel is regulated by ABA kinase Pd OST1. Energy-dispersive X-ray analysis ( EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl− medium, Pd OST1 did not activate the desert plant anion channel Pd SLAC1 per se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated Pd SLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure., Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium.
- Subjects
DATE palm; STOMATA; CELL communication; DESERT plants -- Adaptation; ABSCISIC acid
- Publication
New Phytologist, 2017, Vol 216, Issue 1, p150
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.14672