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- Title
Evolutionary and molecular analysis of Dof transcription factors identified a conserved motif for intercellular protein trafficking.
- Authors
Chen, Huan; Ahmad, Munawar; Rim, Yeonggil; Lucas, William J.; Kim, Jae‐Yean
- Abstract
Cell-to-cell trafficking of transcription factors ( TFs) has been shown to play an important role in the regulation of plant developmental events, but the evolutionary relationship between cell-autonomous and noncell-autonomous ( NCA) TFs remains elusive., At Dof4.1, named INTERCELLULAR TRAFFICKING DOF 1 ( ITD1), was chosen as a representative NCA member to explore this evolutionary relationship. Using domain structure-function analyses and swapping studies, we examined the cell-to-cell trafficking of plant-specific Dof TF family members across Arabidopsis and other species., We identified a conserved intercellular trafficking motif ( ITM) that is necessary and sufficient for selective cell-to-cell trafficking and can impart gain-of-function cell-to-cell movement capacity to an otherwise cell-autonomous TF. The functionality of related motifs from Dof members across the plant kingdom extended, surprisingly, to a unicellular alga that lacked plasmodesmata. By contrast, the algal homeodomain related to the NCA KNOX homeodomain was either inefficient or unable to impart such cell-to-cell movement function., The Dof ITM appears to predate the evolution of selective plasmodesmal trafficking in the plant kingdom, which may well have acted as a molecular template for the evolution of Dof proteins as NCA TFs. However, the ability to efficiently traffic for KNOX homeodomain ( HD) proteins may have been acquired during the evolution of early nonvascular plants.
- Subjects
TRANSCRIPTION factors; PLANT development; NONCELLULOSE organic fiber; ARABIDOPSIS; CELL motility
- Publication
New Phytologist, 2013, Vol 198, Issue 4, p1250
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.12223