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- Title
Prevalence of Severe Acute Respiratory Syndrome Coronavirus 2 Neutralizing Antibodies in Egyptian Convalescent Plasma Donors.
- Authors
Gomaa, Mokhtar R.; Kandeil, Ahmed; Mostafa, Ahmed; Roshdy, Wael H.; Kayed, Ahmed E.; Shehata, Mahmoud; Kutkat, Omnia; Moatasim, Yassmin; El Taweel, Ahmed; Mahmoud, Sara H.; Kamel, Mina Nabil; Abo Shama, Noura M.; El Sayes, Mohamed; El-Shesheny, Rabeh; Bakheet, Osama H.; Elgohary, Mohamed A.; Elbadry, Mohamed; Nassif, Naguib N.; Ahmed, Salwa H.; Abdel Messih, Ibrahim Y.
- Abstract
Using convalescent plasma as immunotherapy is an old method for treatment of infectious diseases. Several countries have recently allowed the use of such therapy for the treatment of COVID-19 patients especially those who are critically ill. A similar program is currently being tested in Egypt. Here, we tested 227 plasma samples from convalescent donors in Egypt for neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using a microneutralization (MN) assay. A third of the tested samples did not have antibody titers and 58% had titers between 1:10 and 1:80. Only 12% had titers >1:160. We also compared MN assays using different virus concentrations, plaque reduction neutralization (PRNT) assays, and a chemiluminescence assay that measures immunoglobulin G (IgG) binding to N and S proteins of SARS-CoV-2. Our results indicated that a MN assay using 100 TCID50/ml provides comparable results to PRNT and allows for high throughput testing.
- Subjects
EGYPT; CONVALESCENT plasma; COVID-19; ANTIBODY titer; IMMUNOGLOBULINS; CHEMILUMINESCENCE assay; IMMUNOGLOBULIN G
- Publication
Frontiers in Microbiology, 2020, Vol 11, pN.PAG
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2020.596851