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- Title
Mechanical-scan-free multicolor super-resolution imaging with diffractive spot array illumination.
- Authors
Xu, Ning; Bohndiek, Sarah E.; Li, Zexing; Zhang, Cilong; Tan, Qiaofeng
- Abstract
Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multicolor imaging methods with high-quality illumination are currently lacking. In this paper, we present for the first time Mechanical-scan-free multiColor Super-resolution Microscopy (MCoSM) with spot array illumination, which enables mechanical-scan-free super-resolution imaging with adjustable resolution and a good effective field-of-view based on spatial light modulators. Through 100–2,500 s super-resolution spot illumination with different effective fields of view for imaging, we demonstrate the adjustable capacity of MCoSM. MCoSM extends existing spectral imaging capabilities through a time-sharing process involving different color illumination with phase-shift scanning while retaining the spatial flexibility of super-resolution imaging with diffractive spot array illumination. To demonstrate the prospects of MCoSM, we perform four-color imaging of fluorescent beads at high resolution. MCoSM provides a versatile platform for studying molecular interactions in complex samples at the nanoscale level. Here, the authors use spot array illumination to enable mechanical-scan-free super-resolution microscopy with adjustable resolution and good effective field of view, demonstrating a platform for studying molecular interactions at the nanoscale.
- Subjects
HIGH resolution imaging; SPECTRAL imaging; LIGHTING; MOLECULAR interactions; SPATIAL light modulators
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-48482-z