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- Title
Centromere defects, chromosome instability, and cGAS-STING activation in systemic sclerosis.
- Authors
Paul, Souren; Kaplan, Mark H.; Khanna, Dinesh; McCourt, Preston M.; Saha, Anjan K.; Tsou, Pei-Suen; Anand, Mahek; Radecki, Alexander; Mourad, Mohamad; Sawalha, Amr H.; Markovitz, David M.; Contreras-Galindo, Rafael
- Abstract
Centromere defects in Systemic Sclerosis (SSc) have remained unexplored despite the fact that many centromere proteins were discovered in patients with SSc. Here we report that lesion skin fibroblasts from SSc patients show marked alterations in centromeric DNA. SSc fibroblasts also show DNA damage, abnormal chromosome segregation, aneuploidy (only in diffuse cutaneous (dcSSc)) and micronuclei (in all types of SSc), some of which lose centromere identity while retaining centromere DNA sequences. Strikingly, we find cytoplasmic "leaking" of centromere proteins in limited cutaneous SSc (lcSSc) fibroblasts. Cytoplasmic centromere proteins co-localize with antigen presenting MHC Class II molecules, which correlate precisely with the presence of anti-centromere antibodies. CENPA expression and micronuclei formation correlate highly with activation of the cGAS-STING/IFN-β pathway as well as markers of reactive oxygen species (ROS) and fibrosis, ultimately suggesting a link between centromere alterations, chromosome instability, SSc autoimmunity, and fibrosis. Fibrosis of the skin plays an important role in scleroderma. Here the authors demonstrate genetic and epigenetic abnormalities at the centromere that affect the replication of the chromosomes, resulting in activation of pathways involved in inflammation and fibrosis
- Subjects
CENTROMERE; SYSTEMIC scleroderma; CHROMOSOMES; CHROMOSOME replication; CHROMOSOME segregation; REACTIVE oxygen species
- Publication
Nature Communications, 2022, Vol 13, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-022-34775-8