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- Title
CEP128 is involved in spermatogenesis in humans and mice.
- Authors
Zhang, Xueguang; Wang, Lingbo; Ma, Yongyi; Wang, Yan; Liu, Hongqian; Liu, Mohan; Qin, Lang; Li, Jinghong; Jiang, Chuan; Zhang, Xiaojian; Shan, Xudong; Liu, Yuliang; Li, Jinsong; Li, Yaqian; Zheng, Rui; Sun, Yongkang; Sun, Jianfeng; Leng, Xiangyou; Liang, Yan; Zhang, Feng
- Abstract
Centrosomal proteins are necessary components of the centrosome, a conserved eukaryotic organelle essential to the reproductive process. However, few centrosomal proteins have been genetically linked to fertility. Herein we identify a homozygous missense variant of CEP128 (c.665 G > A [p.R222Q]) in two infertile males. Remarkably, male homozygous knock-in mice harboring the orthologous CEP128R222Q variant show anomalies in sperm morphology, count, and motility. Moreover, Cep128 knock-out mice manifest male infertility associated with disrupted sperm quality. We observe defective sperm flagella in both homozygous Cep128 KO and KI mice; the cilia development in other organs is normal—suggesting that CEP128 variants predominantly affected the ciliogenesis in the testes. Mechanistically, CEP128 is involved in male reproduction via regulating the expression of genes and/or the phosphorylation of TGF-β/BMP-signalling members during spermatogenesis. Altogether, our findings unveil a crucial role for CEP128 in male fertility and provide important insights into the functions of centrosomal proteins in reproductive biology. CEP128 is a centrosomal protein important for the organization of centriolar microtubules. Here, the authors show that a CEP128 variant observed in human male siblings causes reduced sperm counts and morphologically abnormal sperm when modeled in mice, suggesting a role for CEP128 in male fertility.
- Subjects
SPERMATOGENESIS; CILIA &; ciliary motion; KNOCKOUT mice; MORPHOGENESIS; MISSENSE mutation; MALE infertility; MICE
- Publication
Nature Communications, 2022, Vol 13, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-022-29109-7