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- Title
Overexpression of ARO10 in pdc5Δmutant resulted in higher isobutanol titers in Saccharomyces cerevisiae.
- Authors
Li, Jingzhi; Feng, Ruiqi; Wen, Zhihui; Zhang, Aili
- Abstract
To investigate effects of different pyruvate decarboxylases on isobutanol titers in Saccharomyces cerevisiae, single-gene deletion of the three PDCs genes encoding pyruvate decarboxylases were constructed in this study. In addition, we over-expressed Ilv2, which catalyzed the first step in the valine synthetic pathway, and Bat2, which was the cytoplasmic branched-chain amino-acid aminotransferase that catalyzed L-valine to 2-ketoisovalerate, to increase isobutanol production in the genetically modified strains. Our results showed that knockout of PDC5 were one of the main factors among the three PDC genes for improving isobutanol titers in S. cerevisiae. Additionally, we found that deletion of PDC5 in strain carrying overexpressed ILV2 and ARO10 resulted in 8-fold higher isobutanol productivity as compared to the control strain in micro-aerobic fermentations. Our results also suggested that engineered strain pdc5ΔpILV2 pARO10 generated lower ethanol titers and higher acetate acid titers than the control strain, while the growth rate and glucose consumption rate of engineered strain pdc5ΔpILV2 pARO10 were slightly lower than that of the control strain. Meanwhile, the biomass concentration of pdc5ΔpILV2 pARO10 decreased dramatically than that of the control strain.
- Subjects
ISOBUTANOL; PYRUVATE decarboxylase; SACCHAROMYCES cerevisiae; FERMENTATION; DELETION mutation
- Publication
Biotechnology & Bioprocess Engineering, 2017, Vol 22, Issue 4, p382
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-017-0028-0