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- Title
THE ASSESSMENT OF ANTIBACTERIAL EFFECT OF 0.1% OCTENIDINE SOLUTION ON IN VITRO BIOFILMS OF STREPTOCOCCUS SALIVARIUS USING BACTERIAL LIVE/DEAD VIABILITY STAINING AND CFU COUNTING.
- Authors
AL-SEBAIE, DERAR
- Abstract
The purpose of this study was to assess the antibacterial effect of 0,1% octenidole solution on Streptococcus salivariusbiofilms when using live/dead staining and standard CFU (colony forming units) counting for determination of the bacterial survival rate. Streptococcus salivarius biofilms were grown in vitro for 42h on 12 mm titanium discs in a flow chamber system. Formed Biofilms were exposed to 0,1% octenidine (Schülke,..) solution for 30s, 60s, 120s or 300s. The bacterial kill rate was determined by plating on TSB agar and CFU counting as well as live/dead viability staining (BacLight Viability Kit, Invitrogen,) and confocal laser scanning microscopy (CLSM) analyses. Using the plating method and CFU counting, complete killing of adherent could be observed after 30s treatment. Live/dead staining showed complete killing of bacteria even after 5 minutes immersion of biofilms in octenidine solution. According to this study, significant differences of bacterial survival rates were observed with the two methods used. Therefore, it was concluded that special care should be taken when choosing a laboratory method for the evaluation of antibacterial effects.
- Subjects
ANTIBACTERIAL agents; BIOFILMS; STREPTOCOCCUS salivarius; MOUTHWASHES; BACTERIAL colonies; PREVENTION; THERAPEUTICS
- Publication
Pakistan Oral & Dental Journal, 2014, Vol 34, Issue 2, p281
- ISSN
1012-8700
- Publication type
Article