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- Title
The effect of different biosecurity, feed, and frequency of breeding on the fecundity and fertility of Zebrafish (Danio rerio).
- Authors
WARMSLEY, JOE; BARWOOD, PAUL; MOICHE, VISILA; WILSON, CAROLE
- Abstract
An investigation into what differences in environments, both intrinsic and extrinsic, make to the success of breeding in Zebrafish (Danio rerio). Four different groups were set up across two different environments. The environmental differences included aquatic systems built by different manufacturers, one utilising a drum filtration system and the other with a more conventional sock filtration system. The room employing drum filtration was also held at a higher level of biosecurity - appearing to be free from Mycobacterium marinum, Mycobacterium haemophilium and without evidence of Pseudoloma neurophilia. The conventional room was older and had fewer biosecurity measures. Both rooms were fed the same dry diet and the more biosecure room fed rotifer as a live feed with the conventional room fed artemia. Two lines of fish were used in this trial - AB and TL and once the fish were sexually mature - 56 days post fertilisation (dpf) they were set up in breeding boxes, at varying time intervals in both rooms. The number and proportion of viable and non-viable embryos were counted to determine the level of fecundity and fertility and the differences between the two rooms and between the frequencies of breeding were also measured. It is hoped these results will help reduce both the number of fish needed and their frequency of use for breeding and improve welfare by reducing handling time while increasing embryo number collection and overall refining the breeding process to become more efficient. Additionally, this trial also highlights potential differences in results between facilities employing different husbandry and welfare practices.
- Subjects
BIOSECURITY; BREEDING; FERTILITY; LOGPERCH; MYCOBACTERIUM marinum
- Publication
Animal Technology & Welfare, 2021, Vol 20, Issue 2, p104
- ISSN
1742-0385
- Publication type
Article