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- Title
Generation of recombinant bioluminescent Escherichia coli for quantitative determination of bacterial adhesion.
- Authors
Rahman AlLuhaybi, Khalid Abdul; Alghaith, Ghadah Yazeed; Moneib, Nayera Ahmed; Magead Yassien, Mahmoud Abdul
- Abstract
Bacterial adhesion to urinary catheter was evaluated by measuring the light emitted from a recombinant bioluminescent glycocalyx producer Escherichia coli strain. Generation of the bioluminescent strain was carried out by transforming the bacterial cells with pUCP18-GFP plasmid that contains a green fluorescence gene. Light emission measurement was closely correlated with the number of the adherent cells, giving a detectable signal from 1.2 X 10² cells. The efficiency of this assay was confirmed by testing the antiadherent effect of subinhibitory concentrations of ciprofloxacin with the aid of a model for in-vitro catheter colonization. There was no significant difference in the percentage reduction of adherent cells obtained by both light emission measurement and viable cell count techniques.
- Subjects
BIOLUMINESCENCE assay; URINARY catheters; ESCHERICHIA coli; RECOMBINANT microorganisms; BACTERIAL adhesion; GLYCOCALYX
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2015, Vol 28, Issue 4, p1301
- ISSN
1011-601X
- Publication type
Article