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- Title
Betulinic Acid Enhances the Sensitivity of Multiple Myeloma Cells to Bortezomib by the Inactivation of the AKT/mTOR Pathway.
- Authors
Dan Yu; Sheng Wan; Lili Hu
- Abstract
This study aimed to investigate the mechanism of betulinic acid on multiple myeloma cell resistance to bortezomib. To this end, the bortezomib-resistant RPMI-8226-R cells were generated by prolonged treatment of RPMI-8226 cells with increasing concentrations of bortezomib. Based on the measurements of cell viability and colony number, RPMI-8226-R cells exhibited enhanced resistance to bortezomib than RPMI-8226 cells. Treatment with betulinic acid resulted in increased sensitivity of RPMI-8226-R to bortezomib. When RPMI-8226-R cells were co-treated with bortezomib and betulinic acid, there was an increase in apoptosis rate, cleaved caspase-3, cleaved caspase-9 expression and the decrease in p-AKT/AKT and p-mTOR/mTOR levels. These results suggest that betulinic acid enhances the sensitivity of RPMI-8226-R cells to bortezomib by inhibiting the activation of the AKT/mTOR pathway in bortezomib-resistant multiple myeloma cells.
- Subjects
APOPTOSIS; CELL physiology; CELLULAR signal transduction; DRUG resistance; GENE expression; MOLECULAR structure; MULTIPLE myeloma; PROTEIN kinases; TRANSFERASES; PLANT extracts; CASPASES; BORTEZOMIB
- Publication
Current Topics in Nutraceutical Research, 2020, Vol 18, Issue 3, p241
- ISSN
1540-7535
- Publication type
Article
- DOI
10.37290/ctnr2641-452X.18:241-246