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- Title
Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility.
- Authors
Sellami, Afifa; Chakroun, Nozha; Zarrouk, Soumaya Ben; Sellami, Hanen; Kebaili, Sahbi; Rebai, Tarek; Keskes, Leila
- Abstract
During spermatogenesis, sperm chromatin undergoes structural changes and results in a high condensation. This nuclear compaction would be useful as a predictor of spermfertilization capacity and pregnancy outcome. We purpose to evaluate firstly the relationship among chromatin maturity assessed by aniline blue staining (AB) and the semen parameters in infertile men. Secondly, we analyzed whether the sperm gradient density centrifugation is effective to select mature spermatozoa. Fifty-one ejaculates were investigated by semen analysis and stained for chromatin condensationwithAB to distinguish between unstainedmature spermand stained immature sperm. AB was applied also on 12 ejaculates which proceeded by density gradient centrifugation to compare the rates of immature sperm before and after selection. Neat semen were divided into two groups: G1 (n = 31): immature sperm <20% and G2 (n = 20): immature sperm =20%. No significant differences were detected in sperm concentration, motility, and normal morphology between G1 and G2. However, the rates of some morphology abnormalities were higher in G2: head abnormalities (P = 0.01) and microcephalic sperm (P = 0.02). We founded significant correlation between sperm immaturity and acrosome abnormalities (r = 0.292; P = 0.03). Spermselection has significantly reduced the rates of immature sperm. A better understanding of chromatin structure and its impact on the sperm potential is needed to explore male infertility.
- Subjects
SPERMATOZOA analysis; CHROMATIN; MALE infertility; SPERMATOGENESIS; DENSITY gradient centrifugation; ACROSOMES; DIAGNOSIS
- Publication
Advances in Urology, 2013, p1
- ISSN
1687-6369
- Publication type
Article
- DOI
10.1155/2013/578631