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- Title
Dexamethasone-Loaded Lipomers: Development, Characterization, and Skin Biodistribution Studies.
- Authors
Pena-Rodríguez, Eloy; Lajarin-Reinares, Maria; Mata-Ventosa, Aida; Pérez-Torras, Sandra; Fernández-Campos, Francisco; Silva, Ana Catarina; Manuel Sousa Lobo, José
- Abstract
Follicular targeting has gained more attention in recent decades, due to the possibility of obtaining a depot effect in topical administration and its potential as a tool to treat hair follicle-related diseases. Lipid core ethyl cellulose lipomers were developed and optimized, following which characterization of their physicochemical properties was carried out. Dexamethasone was encapsulated in the lipomers (size, 115 nm; polydispersity, 0.24; zeta-potential (Z-potential), +30 mV) and their in vitro release profiles against dexamethasone in solution were investigated by vertical diffusion Franz cells. The skin biodistribution of the fluorescent-loaded lipomers was observed using confocal microscopy, demonstrating the accumulation of both lipomers and fluorochromes in the hair follicles of pig skin. To confirm this fact, immunofluorescence of the dexamethasone-loaded lipomers was carried out in pig hair follicles. The anti-inflammatory (via TNFα) efficacy of the dexamethasone-loaded lipomers was demonstrated in vitro in an HEK001 human keratinocytes cell culture and the in vitro cytotoxicity of the nanoformulation was investigated.
- Subjects
ETHYLCELLULOSE; SKIN permeability; HUMAN cell culture; TOPICAL drug administration; HAIR follicles; HAIR diseases
- Publication
Pharmaceutics, 2021, Vol 13, Issue 4, p533
- ISSN
1999-4923
- Publication type
Article
- DOI
10.3390/pharmaceutics13040533