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- Title
Structural Change in Microbiota by a Probiotic Cocktail Enhances the Gut Barrier and Reduces Cancer via TLR2 Signaling in a Rat Model of Colon Cancer.
- Authors
Kuugbee, Eugene; Shang, Xueqi; Gamallat, Yaser; Bamba, Djibril; Awadasseid, Annoor; Suliman, Mohammed; Zang, Shizhu; Ma, Yufang; Chiwala, Gift; Xin, Yi; Shang, Dong; Kuugbee, Eugene Dogkotenge; Suliman, Mohammed Ahmed
- Abstract
<bold>Background: </bold>Structural change in the gut microbiota is implicated in cancer. The beneficial modulation of the microbiota composition with probiotics and prebiotics prevents diseases.<bold>Aim: </bold>We investigated the effect of oligofructose-maltodextrin-enriched Lactobacillus acidophilus, Bifidobacteria bifidum, and Bifidobacteria infantum (LBB), on the gut microbiota composition and progression of colorectal cancer.<bold>Methods: </bold>Sprague Dawley rats were acclimatized, given ampicillin (75 mg/kg), and treated as follows; GCO: normal control; GPR: LBB only; GPC: LBB+ 1,2-dimethylhydrazine dihydrochloride (DMH); and GCA: DMH only (cancer control). 16S V4 Pyrosequencing for gut microbiota analysis, tumor studies, and the expression of MUC2, ZO-1, occludin, TLR2, TLR4, caspase 3, COX-2, and β-catenin were conducted at the end of experiment.<bold>Results: </bold>Probiotic LBB treatment altered the gut microbiota. The relative abundance of genera Pseudomonas, Congregibacter, Clostridium, Candidactus spp., Phaeobacter, Escherichia, Helicobacter, and HTCC was decreased (P < 0.05), but the genus Lactobacillus increased (P < 0.05), in LBB treatment than in cancer control. The altered gut microbiota was associated with decreased tumor incidence (80 % in GPC vs. 100 % in GCA, P = 0.0001), tumor volume (GPC 84.23 (42.75-188.4) mm(3) vs. GCA 243 (175.5-344.5) mm(3), P < 0.0001) and tumor multiplicity/count (GPC 2.92 ± 0.26 vs. GCA 6.27 ± 0.41; P < 0.0001). The expression of MUC2, ZO-1, occludin, and TLR2 was increased, but expression of TLR4, caspase 3, Cox-2, and β-catenin was decreased by LBB treatment than in cancer control GCA (P < 0.05).<bold>Conclusion: </bold>Administration of LBB modulates the gut microbiota and reduces colon cancer development by decreasing tumor incidence, multiplicity/count, and volume via enhanced TLR2-improved gut mucosa epithelial barrier integrity and suppression of apoptosis and inflammation.
- Subjects
COLON cancer prevention; GUT microbiome; PROBIOTICS; COCKTAILS; TOLL-like receptors; CELLULAR signal transduction; LABORATORY rats; COLON microbiology; ANIMAL experimentation; ANTHROPOMETRY; BIOLOGICAL models; CARCINOGENS; CELL receptors; COLON (Anatomy); COLON tumors; GLYCOPROTEINS; IMMUNOHISTOCHEMISTRY; LACTOBACILLUS acidophilus; OXIDOREDUCTASES; POLYMERASE chain reaction; RATS; TUMORS; DISEASE progression
- Publication
Digestive Diseases & Sciences, 2016, Vol 61, Issue 10, p2908
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-016-4238-7