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- Title
Allograft rejection in athymic nude rats by transferred T-cell subsets I. THE RESPONSE OF NAIVE CD4<sup>+</sup> AND CD8<sup>+</sup> THORACIC DUCT LYMPHOCYTES TO COMPLETE ALLOGENIC INCOMPATIBILITIES.
- Authors
Whitby, E. H.; Sparshott, S. M.; Bell, E. B.
- Abstract
PVG.rnu/rnu nude rats were pie-grafted with two allogeneic skin grafts, AO(RTIu) and BN(RTIn), 6-14 days in advance of cell transfer. Cellular requirements for rejection were established by transferring graded numbers of B cell-depleted (lg-) thoracic duct lymphocytes (TDL) or purified W3/25+ (CD4+) or 0X8 (CD8+)TDL subsets. Allografts were rejected by 105 to 5 x 106 Ig TDL in a dose-dependent fashion. A similar dose-response relationship was found by transferring 5 x 105 to 5 x 106 Ig-OX8 TDL (purified by depletion of B cells and 0X8+ cells). Larger numbers of lg-0X8- TDL (10-30 x 106) did not significantly accelerate rejection. W3/25+ TDL alone (l05), highly purified by fluorescence-activated cell sorting (FACS), were sufficient to induce allograft rejection in this athymic nude rat model. In contrast, 10 times more FACS purified 0X8+ TDL (106) were unable to initiate skin graft rejection despite the complete class I and class II MHC incompatibilities. Furthermore, the addition of 106 0X8+ cells did not accelerate or retard the rejection induced by 105 W3/25+ cells alone. Pre-grafted nude recipients, irradiated (500 R) 2 hr before W3/25+ TDL injection, in order to eliminate putative nude T cells, rejected allografts on the same day as unirradiated controls. We conclude that when confronted with complete MHC disparities, CD4+ T cells are necessary and sufficient to induce skin allograft rejection whereas CD8+ T cells do not appear to contribute.
- Subjects
HOMOGRAFTS; TRANSPLANTATION of organs, tissues, etc.; LYMPHOCYTES; BIOCHEMISTRY; TRANSPLANTATION immunology; T cells
- Publication
Immunology, 1990, Vol 69, Issue 1, p78
- ISSN
0019-2805
- Publication type
Article