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- Title
Inclusion Bodies Contraction with Implications in Biotechnology.
- Authors
Peternel, Špela; Bele, Marjan; Gaberc-Porekar, Vladka; Komel, Radovan
- Abstract
Irreversible contraction of inclusion bodies was found to have a huge impact on sedimentation velocity, solubility and extractability of target protein from non-classical inclusion bodies. The downstream process during protein isolation from non-classical IBs is also affected. Based on extractability studies accompanied by electron microscopy, a hypothetical model of IBs formation mechanism is presented, which is supported by usually observed single IB per E. coli cell.
- Subjects
SEDIMENTATION &; deposition; SOLUBILITY; ELECTRON microscopy; PROTEINS; BIOTECHNOLOGY
- Publication
Acta Chimica Slovenica, 2008, Vol 55, Issue 3, p608
- ISSN
1318-0207
- Publication type
Article