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- Title
Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line.
- Authors
HAO TAO; FENG CHEN; HAIFEI LIU; YANLING HU; YINGZHEN WANG; HAIYAN LI
- Abstract
Anaberrant Wnt/β-catenin signaling pathway is frequently implicated in tumorigenesis. However, whether the Wnt/β-catenin pathway plays a role in resistance to antitumor chemotherapy drugs remains unknown. In the present study, the process of autophagy was assessed following overexpression of the autophagy-associated gene Beclin 1 in gemcitabine-induced MG63 human osteosarcoma cells. Autophagy-associated gene expression was measured following activation or inhibition of the Wnt/β-catenin pathway in gemcitabine-induced MG63 cells using reverse transcription-quantitative polymerase chain reaction. In addition, the percentage of MG63 cell apoptosis was measured by flow cytometry following Wnt/β-catenin pathway activation or inhibition. The results demonstrated that Beclin 1 overexpression induced autophagy and reduced gemcitabine-induced apoptosis in MG63 human cell line. Furthermore, activation of the Wnt/β-catenin signaling pathway attenuated autophagy and enhanced gemcitabine-induced apoptosis. Additionally, the expression of Beclin 1 was reduced following Wnt/β-catenin signaling pathway activation. The present study demonstrated that activation of the Wnt/β-catenin signaling pathway may rescue chemotherapy drug resistance by downregulating the expression of Beclin 1.
- Subjects
WNT signal transduction; AUTOPHAGY; OSTEOSARCOMA; CELL lines; GENE expression
- Publication
Molecular Medicine Reports, 2017, Vol 16, Issue 2, p1701
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2017.6828