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- Title
Shensongyangxin protects against pressure overload-induced cardiac hypertrophy.
- Authors
DI-FEI SHEN; QING-QING WU; JIAN NI; WEI DENG; CONG WEI; ZHEN-HUA JIA; HENG ZHOU; MENG-QIAO ZHOU; ZHOU-YAN BIAN; QI-ZHU TANG
- Abstract
Shensongyangxin (SSYX) is a medicinal herb, which has long been used in traditional Chinese medicine. Various pharmacological activities of SSYX have been identified. However, the role of SSYX in cardiac hypertrophy remains to be fully elucidated. In present study, aortic banding (AB) was performed to induce cardiac hypertrophy in mice. SSYX (520 mg/kg) was administered by daily gavage between 1 and 8 weeks following surgery. The extent of cardiac hypertrophy was then evaluated by pathological and molecular analyses of heart tissue samples. In addition, in vitro experiments were performed to confirm the in vivo results. The data of the present study demonstrated that SSYX prevented the cardiac hypertrophy and fibrosis induced by AB, as assessed by measurements of heart weight and gross heart size, hematoxylin and eosin staining, cross-sectional cardiomyocyte area and the mRNA expression levels of hypertrophic markers. SSYX also inhibited collagen deposition and suppressed the expression of transforming growth factor β (TGFβ), connective tissue growth factor, fibronectin, collagen ?α and collagen ?α, which was mediated by the inhibition of the TGFβ/small mothers against decapentaplegic (Smad) signaling pathway. The inhibitory action of SSYX on cardiac hypertrophy was mediated by the inhibition of Akt signaling. In vitro investigations in the rat H9c2 cardiac cells also demonstrated that SSYX attenuated angiotensin II-induced cardiomyocyte hypertrophy. These findings suggested that SSYX attenuated cardiac hypertrophy and fibrosis in the pressure overloaded mouse heart. Therefore, the cardioprotective effect of SSYX is associated with inhibition of the Akt and TGFβ/Smad signaling pathways.
- Subjects
HERBAL medicine; CARDIAC hypertrophy; PHARMACOLOGY; TRANSFORMING growth factors; DECAPENTAPLEGIC protein; LABORATORY mice; PREVENTION
- Publication
Molecular Medicine Reports, 2016, Vol 13, Issue 1, p980
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2015.4598