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- Title
l-Ribose Production from l-Arabinose by Immobilized Recombinant Escherichia coli Co-expressing the l-Arabinose Isomerase and Mannose-6-Phosphate Isomerase Genes from Geobacillus thermodenitrificans.
- Authors
Kim, Kyoung-Rok; Seo, Eun-Sun; Oh, Deok-Kun
- Abstract
l-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the l-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The immobilized cells produced 99 g/l l-ribose from 300 g/l l-arabinose in 3 h at pH 7.5 and 60 °C in the presence of 1 mM Co, with a conversion yield of 33 % ( w/ w) and a productivity of 33 g/l/h. The immobilized cells in the packed-bed bioreactor at a dilution rate of 0.2 h produced an average of 100 g/l l-ribose with a conversion yield of 33 % and a productivity of 5.0 g/l/h for the first 12 days, and the operational half-life in the bioreactor was 28 days. Our study is first verification for l-ribose production by long-term operation and feasible for cost-effective commercialization. The immobilized cells in the present study also showed the highest conversion yield among processes from l-arabinose as the substrate.
- Subjects
RIBOSE; RECOMBINANT microorganisms; ARABINOSE; ESCHERICHIA coli; ISOMERASES; GENE expression in bacteria; MANNOSE 6-phosphate; BACILLUS (Bacteria)
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 172, Issue 1, p275
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-013-0547-x