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- Title
Identification of a TBHQ-Interfering Peak in Crude Canola Oil Using AOCS Official Method Ce 6-86 and its Chromatographic Resolution.
- Authors
Blumhorst, Michael R.; Mahan, Travis; Stanley, Kathryn; Griffith, Aaron; Collison, Mark W.
- Abstract
AOCS Official Method Ce 6-86 'Antioxidants, Liquid Chromatographic Method' was originally developed to confirm the correct antioxidant was added at the specified concentration to refined oils. Today, this method is increasingly utilized to validate that antioxidants are absent from oil products. False positive results can have a significant impact on the ability to sell products in specific markets and can impart additional business expenditures for conclusive secondary analyses. In the current work, quantification of tert-butylhydroquinone (TBHQ) in crude canola/rapeseed oil using liquid chromatography (LC) with ultraviolet (UV) detection was compromised by an interfering peak. Analyses using liquid chromatography-mass spectrometry (GC-MS) and high-resolution accurate mass LC-MS identified the interferent as 2,6-dimethoxy-4-vinylphenol (canolol), an endogenous compound present in crude canola/rapeseed oil. Resolution of canolol and TBHQ using LC-UV can be achieved via minor modification of the chromatographic conditions.
- Subjects
VEGETABLE oil analysis; CANOLA oil; LIQUID chromatography; ULTRAVIOLET spectra; AMERICAN Oil Chemists' Society (Organization); CHARTS, diagrams, etc.; COOKING
- Publication
Journal of the American Oil Chemists' Society (JAOCS), 2017, Vol 94, Issue 10, p1323
- ISSN
0003-021X
- Publication type
Article
- DOI
10.1007/s11746-017-3039-2