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- Title
Bio‐probing with nonresonant surface‐enhanced hyper‐Raman scattering excited at 1,550 nm.
- Authors
Heiner, Zsuzsanna; Madzharova, Fani; Živanović, Vesna; Kneipp, Janina
- Abstract
The two‐photon excited process of surface‐enhanced hyper‐Raman scattering (SEHRS) provides advantages for studies of complex biological samples, yet suitable SEHRS nanoprobes and labels, as well as experimental conditions, must be established. Here, SEHRS spectra of the four reporter molecules (2‐naphthalenethiol [2‐NAT], para‐aminothiophenol (pATP), para‐nitrothiophenol (pNTP), and crystal violet), as well as the two antidepressant drugs (desipramine and imipramine) were obtained at an excitation wavelength of 1,550 nm using different citrate‐stabilized gold nanoparticles and silver nanoparticles, and under conditions that permit experiments with living cultured cells. As the results suggest, the short‐wave infrared laser excitation and the hyper‐Raman scattered light (corresponding to wavelengths > 775 nm) match the plasmonic properties of the employed gold and silver nanoaggregates, as well as the requirements regarding the viability of the cells. The two‐photon excited spectra of three types of SEHRS labels containing 2‐NAT as reporter inside macrophage cells show that molecules in the cellular environment can also be observed. The possibility to use short‐wave infrared excitation with gold nanostructures has important implications for the utilization of SEHRS in bio‐probing.
- Subjects
GOLD nanoparticles; SILVER nanoparticles; INFRARED lasers; GENTIAN violet; LIGHT scattering; BIOLUMINESCENCE; SURFACE enhanced Raman effect
- Publication
Journal of Raman Spectroscopy, 2021, Vol 52, Issue 2, p394
- ISSN
0377-0486
- Publication type
Article
- DOI
10.1002/jrs.5965