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- Title
Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin.
- Authors
Heinzmann, Stefan; Entian, Karl-Dieter; Stein, Torsten
- Abstract
To improve the production of the lantibiotic subtilin in Bacillus subtilis ATCC 6633, two genetic engineering strategies were followed. Firstly, additional copies of subtilin self-protection (immunity) genes spaIFEG have been integrated into the genome of the producer strain. Their expression significantly enhanced the subtilin tolerance level, and concomitantly, the subtilin yield 1.7-fold. Secondly, a repressor of subtilin gene expression, the B. subtilis general transition state regulator protein AbrB, was deleted. A sixfold enhancement of the subtilin yield could be achieved with the abrB deletion mutant; however, the produced subtilin fraction predominantly consists of succinylated subtilin species with less antimicrobial activity compared to unmodified subtilin.
- Subjects
SUBTILIN; BACILLUS subtilis; GENETIC engineering; IMMUNITY; GENOMES
- Publication
Applied Microbiology & Biotechnology, 2006, Vol 69, Issue 5, p532
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-005-0023-9