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- Title
PPX1 gene overexpression has no influence on polyphosphates in Saccharomyces cerevisiae.
- Authors
Lichko, L.; Eldarov, M.; Dumina, M.; Kulakovskaya, T.
- Abstract
The role of exopolyphosphatase PPX1 in polyphosphate metabolism in yeasts has been studied in strains of Saccharomyces cerevisiae with inactivated PPX1 and PPN1 genes transformed by the expression vector carrying the yeast PPX1 gene. Exopolyphosphatase activity in transformant strains increased 90- and 40-fold compared to the ΔPPX1 and ΔPPN1 strains, respectively. The purified recombinant exopolyphosphatase PPX1 was similar to the PPX1 of wild strains in its substrate specificity and requirement for divalent metal cations. It was more active with tripolyphosphate and low molecular mass polyphosphates than with high molecular mass polyphosphates and required Mg for its activity. The high level of recombinant PPX1 expression caused no decrease in polyphosphate content in the cells of the transformant. This fact suggests the restricted role of PPX1 in polyphosphate metabolism in yeasts.
- Subjects
EXOPOLYPHOSPHATASE; SACCHAROMYCES cerevisiae; GENETIC overexpression; FUNGAL metabolism; GENETIC transformation; RECOMBINANT proteins; MOLECULAR weights
- Publication
Biochemistry (00062979), 2014, Vol 79, Issue 11, p1211
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S000629791411008X