We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes.
- Authors
Peina Zhou; Hongyu Chen; Jingjie Dang; Zunrui Shi; Yongfang Shao; Chanchan Liu; Longjiang Fan; Qinan Wu
- Abstract
The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were used to isolated protoplasts and successfully captured 33,254 protoplasts for sequencing purposes. After cell type annotation, all the cells were partitioned into six broad populations with 19 clusters. Cells from PGTs were identified based on the expression patterns of trichome-specific genes, monoterpene biosynthetic genes, and metabolic analysis of PGT secretions. The developmental trajectories of PGTs were delineated by pseudotime analysis. Integrative analysis of scRNA-seq data from N. tenuifolia leaves and Arabidopsis thaliana shoot revealed that PGTs were specific to N. tenuifolia. Thus, our results provide a promising basis for exploring cell development and differentiation in plants, especially glandular trichome initiation and development.
- Subjects
PLANT cell development; NEPETA; PLANT cell differentiation; TRICHOMES; TRANSCRIPTOMES; PLANT protoplasts
- Publication
Frontiers in Plant Science, 2022, Vol 13, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2022.988594