We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Anti-inflammatory secondary metabolites from Scrophularia kotschyana.
- Authors
Renda, Gülin; Kadıoğlu, Mine; Kılıç, Merve; Korkmaz, Büşra; Kırmızıbekmez, Hasan
- Abstract
The species belonging to Scrophularia genus grow mainly in Irano-Turanian and Mediterranean regions and have been used as folk remedy for inflammatory-related diseases since ancient times. The present study was aimed to evaluate the anti-inflammatory activity of the extracts of Scrophularia kotschyana as well as the isolated compounds. The aerial parts and the roots of the plant were separately extracted with methanol. Anti-inflammatory activities of both extracts were evaluated with formalin test in mice. As the methanolic extract of the aerial parts significantly (p <.05) inhibited inflammation, it was then submitted to successive solvent extractions with n -hexane, dichloromethane, ethyl acetate and n -butanol to yield subextracts. Anti-inflammatory activities of the subextracts were evaluated within the same test system. Among the subextracts tested, the n -butanol subextract produced a significant (p <.05) anti-inflammatory activity at all doses (5, 10, and 30 mg/kg, ip.). Sequential chromatographic separation of the n -butanol subextract yielded 8-O-acetyl-4′- O -(E)- p -coumaroylharpagide, 8- O -acetyl-4′- O -(Z)- p -coumaroylharpagide, β-sitosterol 3- O -β-glucopyranoside, apigenin 7- O -β-glucopyranoside, apigenin 7- O -rutinoside, luteolin 7- O -β-glucopyranoside and luteolin 7- O -rutinoside. The anti-inflammatory activities of the isolates were evaluated at 5 mg/kg dose. Luteolin 7- O -β-glucopyranoside and apigenin 7- O -rutinoside caused a significant (p <.05) inhibition of oedema formation.
- Subjects
METABOLITES; APIGENIN; SOLVENT extraction; ETHYL acetate; FLAVONOID glycosides; LUTEOLIN
- Publication
Human & Experimental Toxicology, 2021, Vol 40, pS676
- ISSN
0960-3271
- Publication type
Article
- DOI
10.1177/09603271211058889