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- Title
Targeted bisulfite sequencing of the dynamic DNA methylome.
- Authors
Ziller, Michael J.; Stamenova, Elena K.; Hongcang Gu; Gnirke, Andreas; Meissner, Alexander
- Abstract
Background: The ability to measure DNA methylation precisely and efficiently continues to drive our understanding of this modification in development and disease. Whole genome bisulfite sequencing has the advantage of theoretically capturing all cytosines in the genome at single-nucleotide resolution, but it has a number of significant practical drawbacks that become amplified with increasing sample numbers. All other technologies capture only a fraction of the cytosines that show dynamic regulation across cell and tissue types. Results: Here, we present a novel hybrid selection design focusing on loci with dynamic methylation that captures a large number of differentially methylated gene-regulatory elements. We benchmarked this assay against matched whole genome data and profiled 25 human tissue samples to explore its ability to detect differentially methylated regions. Results: Here, we present a novel hybrid selection design focusing on loci with dynamic methylation that captures a large number of differentially methylated gene-regulatory elements. We benchmarked this assay against matched whole genome data and profiled 25 human tissue samples to explore its ability to detect differentially methylated regions.
- Subjects
NUCLEOTIDE sequencing; DNA methylation; LOCUS (Genetics); HUMAN genome; GENE regulatory networks; SINGLE nucleotide polymorphisms
- Publication
Epigenetics & Chromatin, 2016, Vol 9, p1
- ISSN
1756-8935
- Publication type
Article
- DOI
10.1186/s13072-016-0105-1