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- Title
RAPD marker analysis of genetic variation in Kluai Nam Wa ( Musa ABB group) banana plants regenerated after 8 and 9 subcultures.
- Authors
Kanjanaphachoat, Parawee; Topoonyanont, Nopmanee; Jantawichai, Parichat
- Abstract
Genetic variation is often found in plantlets propagated from in vitro cultures. In this study striped laminas were observed on Kluai Nam Wa (Musa ABB group) banana plantlets propagated in a temporary immersion bioreactor (TIB) after the eighth and ninth subcultures. For phenotypic confirmation, banana plants with altered morphology were further observed by transplanting in the greenhouse and observing new leaves generated thereafter. Twenty-one random amplified polymorphic DNA (RAPD) primers were used to screen the regenerated bananas for genetic variation. Six random primers were selected for confirming genetic stability between subcultures. Out of the 6 primers used, two revealed polymorphism, showing distinctly different patterns of striped laminas. OPA-14 primer presented a polymorphic pattern in one band (25%) in the eighth subculture plants and in three bands (50%) in the ninth subculture plants. OPD-07 primer showed polymorphism (16.7%) with striped laminas in both the eighth and ninth subcultures. The presence of specific bands in the regenerated bananas of different subcultures indicated that somaclonal variation in the regenerated plants increased with the number of subcultures. Morphological studies showed that the mean height of bananas with striped laminas was lower than that of normal plants when grown under field conditions for 6 months. The appearance of the striped phenotype of regenerated Kluai Nam Wa banana remained stable. Under these conditions, a maximum of 7 subcultures is recommended on account of possible somaclonal variation after 8 subcultures.
- Subjects
GENETIC markers; REGENERATION (Botany); BANANAS; SUBCULTURES; PLANT morphology; PLANT capacity; PLANT variation
- Publication
Maejo International Journal of Science & Technology, 2020, Vol 14, Issue 2, p130
- ISSN
1905-7873
- Publication type
Article