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- Title
Evaluation of characteristic of human turbinate derived mesenchymal stem cells cultured in the serum free media.
- Authors
Hwang, Se Hwan; Lee, WeonSun; Park, Sang Hi; Lee, Hee Jin; Park, Sun Hwa; Lee, Dong Chang; Lim, Mi Hyun; Back, Sang A.; Yun, Byeong Gon; Jeun, Jung Ho; Lim, Jung Yeon; Kang, Jun Myung; Kim, Sung Won
- Abstract
We evaluated the effect of serum-free and xeno-cultivation (SFXFM) on the characterization, proliferation, and differentiation properties of human nasal stem cells (airway tissue; hTMSCs). hTMSCs were isolated from 10 patients, after which patient samples were separated into two groups, an SFXFM group and a control group. The control group was treated with bovine serum-containing medium. FACS analysis revealed that SFXFM-cultured hTMSCs maintained a characteristic mesenchymal stem cell phenotype. hTMSC proliferation was not influenced by SFXFM. In addition, upregulation of IL-8 and GM-CSF and downregulation of RANTES expression were shown in response to SFXFM. Moreover, two-lineage differentiation properties (osteocyte and adipocyte) of hTMSCs were enhanced under SFXFM. Finally, the genetic stability of SFXFM-cultured hTMSCs was demonstrated by normal karyotype results. SFXFM enables good expansion, multipotentiality, and normal genotype maintenance of MSCs. Moreover, this approach serves as a substitute to conventional media for the cultivation of capable MSCs for upcoming medical applications.
- Subjects
TURBINATE bones; MESENCHYMAL stem cells; STEM cell culture; SERUM; KARYOTYPES
- Publication
PLoS ONE, 2017, Vol 12, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0186249