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- Title
The number of replicates, and pooling versus individual measurements for analytical imprecision calculations: Does it matter?
- Authors
Tecles, Fernando; Muñoz, Alberto; Cerón, José J; Freeman, Kathleen
- Abstract
Background: Recommendations from the American Society of Veterinary Clinical Pathology (ASVCP) are to calculate the between‐run coefficient of variation (CV) based on measuring one replicate per day on quality control materials (QCMs) or pooled patient samples over a minimum of 20 days. However, this recommendation is not always followed by researchers. Objectives: We aimed to determine if a reduction in the number of replicates using QCM or individual or pooled samples would provide CV results similar to those obtained based on ASVCP recommendations. Methods: CVs were calculated for three measurands, namely urea, creatinine, and C‐reactive protein based on the analytic results of the following groups: (a) QCM measured once daily for 20 days (considered as the reference for comparison), b) QCM measured once daily for 5 days, (c) five different canine serum samples measured once daily for 5 days, and (d) a pooled canine serum measured once daily for 5 days. CVs were calculated for two different measurand concentrations. Results: Compared with the reference method, significantly different CVs were obtained with all methods except for when the QCM was measured once daily for 5 days. The use of the five different individual samples also provided significantly different CVs compared with the use of a pooled sample. Conclusions: The results indicate that different protocols for determining between‐run imprecision calculations can give different results compared with the reference procedure and that this should be taken into consideration when evaluating the total error associated with a test.
- Subjects
UREA; VETERINARY clinical pathology; C-reactive protein; QUALITY control
- Publication
Veterinary Clinical Pathology, 2020, Vol 49, Issue 1, p112
- ISSN
0275-6382
- Publication type
Article
- DOI
10.1111/vcp.12830