We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors.
- Authors
Wang, Xiaoling; Wang, Yebo; Wu, Xiwei; Wang, Jinhui; Wang, Yingjia; Qiu, Zhaojun; Chang, Tammy; Huang, He; Lin, Ren-Jang; Yee, Jiing-Kuan
- Abstract
The utility of CRISPR-Cas9 and TALENs for genome editing may be compromised by their off-target activity. We show that integrase-defective lentiviral vectors (IDLVs) can detect such off-target cleavage with a frequency as low as 1%. In the case of Cas9, we find frequent off-target sites with a one-base bulge or up to 13 mismatches between the single guide RNA (sgRNA) and its genomic target, which refines sgRNA design.
- Subjects
GENOME editing; GENE expression; DNA damage; RNA sequencing; LENTIVIRUSES
- Publication
Nature Biotechnology, 2015, Vol 33, Issue 2, p175
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt.3127