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- Title
Endothelin-1 Attenuates Apoptosis in Cultured Trophoblasts From Term Human Placentas.
- Authors
Cervar-Zivkovic, M.; Hu, C.; Barton, A.; Sadovsky, Y.; Desoye, G.; Lang, U.; Nelson, D. M.
- Abstract
The authors test the hypothesis that endothelin-1 (ET-1) modulates apoptosis in human term trophoblasts. Primary cultures of cytotrophoblasts from term human placentas (n = 5) were cultured for 16 hours total or 24 hours prior to harvest at 72 hours in atmospheres of <1%, 8%, and 20% oxygen, in the presence of 10% serum, ET-1 (1-100 pmol/mL), both, or neither. The apoptotic cleavage products of poly-ADP-riboose polymerase and cytokeratin 18 filaments were quantified by Western analysis and immunocytochemistry. The expression of BAD, pBAD-serine112, p53, and 2 isoforms immunocytochemistry. Compared to vehicle control, increasing concentrations of ET-1 reduce by 3- to 6-fold the level of apoptosis in cytotrophoblasts exposed to serum-free conditions at 20% oxygen. Similarly, syncytiotrophoblast cultures grown for 24 hours without serum in 100 pmol/mL ET-1 show a 3-fold lower level of apoptosis compared with vehicle control. ET-1 significantly reduces apoptosis in cultures exposed to 20% oxygen in accompanied by reduced p53 expression and is correlated with enhanced expression of endothelin B receptor, compared to cultures in 8% or 1% oxygen. ET-1 reduces apoptosis in cultured human trophoblasts, and this finding suggests a role for ET-1 in protecting trophoblasts against injury.
- Subjects
ENDOTHELINS; APOPTOSIS; IMMUNOCYTOCHEMISTRY; SERUM; CELL receptors
- Publication
Reproductive Sciences, 2007, Vol 14, Issue 5, p430
- ISSN
1933-7191
- Publication type
Article
- DOI
10.1177/1933719107305801