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- Title
Electrical pulse stimulation of cultured skeletal muscle cells as a model for in vitro exercise - possibilities and limitations.
- Authors
Nikolić, N.; Görgens, S. W.; Thoresen, G. H.; Aas, V.; Eckel, J.; Eckardt, K.
- Abstract
The beneficial health-related effects of exercise are well recognized, and numerous studies have investigated underlying mechanism using various in vivo and in vitro models. Although electrical pulse stimulation ( EPS) for the induction of muscle contraction has been used for quite some time, its application on cultured skeletal muscle cells of animal or human origin as a model of in vitro exercise is a more recent development. In this review, we compare in vivo exercise and in vitro EPS with regard to effects on signalling, expression level and metabolism. We provide a comprehensive overview of different EPS protocols and their applications, discuss technical aspects of this model including critical controls and the importance of a proper maintenance procedure and finally discuss the limitations of the EPS model.
- Subjects
SKELETAL muscle; MUSCLE contraction; NEURAL stimulation; METABOLISM; EXERCISE
- Publication
Acta Physiologica, 2017, Vol 220, Issue 3, p310
- ISSN
1748-1708
- Publication type
Article
- DOI
10.1111/apha.12830