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- Title
Fine genetic mapping of the white immature fruit color gene w to a 33.0-kb region in cucumber ( Cucumis sativus L.).
- Authors
Liu, Hanqiang; Meng, Huanwen; Pan, Yupeng; Liang, Xinjing; Jiao, Jianqing; Li, Yuhong; Chen, Shuxia; Cheng, Zhihui
- Abstract
Key message: The white immature fruit color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase. Abstract: The skin color of immature fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC population and two F segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature fruit color genes in cucumbers. Consequently, a single recessive gene, w, was identified that controls white immature fruit color. A total of 526 markers, which were derived from published genetic maps, two reference cucumber genomes ('9930' and GY14), and two parents (Q30 and Q24) for which whole-genome sequence information is available, were used to map the target gene w to a 33.0-kb region flanked by two SNP-based markers, ASPCR39262 and ASPCR39229, which are physically located at 39262450 and 39229482 of chromosome 3 ('9930' draft genome assembly), respectively. Gene prediction indicated that four potential genes were located in the target region. One gene that encodes peroxidase is likely to be a valuable candidate gene because quantitative real-time PCR revealed an eightfold difference in its transcriptional level, and several amino acid variations were found when the deduced amino acid sequence was aligned. A co-segregating marker was used synergistically to test its ability to predict the skin colors of 83 dark green/white germplasms, and the validity of its utility in marker-assisted selection was confirmed. Fine mapping of this locus will assist in cloning the gene and in marker-assisted breeding to develop dark green/white cucumber cultivars.
- Subjects
CUCUMBER genetics; COLOR of fruit; PLANT gene mapping; PLANT germplasm; PEROXIDASE
- Publication
Theoretical & Applied Genetics, 2015, Vol 128, Issue 12, p2375
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-015-2592-z