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- Title
The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans.
- Authors
Zhang, Chunzhi; Liu, Lei; Wang, Xiaoxuan; Vossen, Jack; Li, Guangcun; Li, Tao; Zheng, Zheng; Gao, Jianchang; Guo, Yanmei; Visser, Richard; Li, Junming; Bai, Yuling; Du, Yongchen
- Abstract
Key message: Ph-3 is the first cloned tomato gene for resistance to late blight and encodes a CC-NBS-LRR protein. Abstract: Late blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance ( R) gene Ph- 3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph- 3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph- 3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph- 3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph- 3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph- 3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph- 3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).
- Subjects
SOLANUM; PLANT proteins; PHYTOPHTHORA infestans; BACTERIAL artificial chromosomes; TOMATO genetics; PLANT gene mapping
- Publication
Theoretical & Applied Genetics, 2014, Vol 127, Issue 6, p1353
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-014-2303-1