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- Title
High macro-collinearity between lima bean ( Phaseolus lunatus L.) and the common bean ( P. vulgaris L.) as revealed by comparative cytogenetic mapping.
- Authors
Almeida, Cícero; Pedrosa-Harand, Andrea
- Abstract
Common bean ( P. vulgaris) and lima bean ( P. lunatus) are the most important crop species from the genus Phaseolus. Both species have the same chromosome number ( 2n = 22) and previous cytogenetic mapping of BAC clones suggested conserved synteny. Nevertheless, karyotype differences were observed, suggesting structural rearrangements. In this study, comparative cytogenetic maps for chromosomes 3, 4 and 7 were built and the collinearity between the common bean and lima bean chromosomes was investigated. Thirty-two markers (30 BACs and 2 bacteriophages) from P. vulgaris were hybridized in situ on mitotic chromosomes from P. lunatus. Nine BACs revealed a repetitive DNA pattern with pericentromeric distribution and 23 markers showed unique signals. Nine of these markers were mapped on chromosome 3, eight on chromosome 4 and six on chromosome 7. The order and position of all analyzed BACs were similar between the two species, indicating a high level of macro-collinearity. Thus, although few inversions have probably altered centromere position in other chromosomes, the main karyotypic differences were associated with the repetitive DNA fraction.
- Subjects
LIMA bean; BACTERIOPHAGES; GENE mapping; CYTOGENETICS; KARYOTYPES; COMPARATIVE studies
- Publication
Theoretical & Applied Genetics, 2013, Vol 126, Issue 7, p1909
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-013-2106-9